Fig. 3
From: The restorative role of annexin A1 at the blood–brain barrier
Localisation by immunofluorescence confocal microscopy of ANXA1 in human foetal forebrain at mid-gestation. a, b Single immunolabelling for ANXA1 (green) shows a high expression of the protein in microglia-like cells and b in venular, endothelial cells. Note in b the prevalent expression of ANXA1 on the luminal side of the endothelial plasma membrane (arrow). c, d Double immunolabelling for ANXA1/collagen IV. c The vascular basal membrane revealed by collagen IV allow to identify the shows ANXA1 reactivity localised on the endothelial lining; d ANXA1-negative pericyte embedded in the basal lamina and the localization of ANXA1 on the endothelial cell nucleus. e, f Double immunolabelling for ANXA1/GFAP shown on a confocal single optical plane. e ANXA1 reactıve endothelial cells in contact with GFAP labelled perivascular astrocyte processes; f a detail of ANXA1 localization on the endothelial membranes. Nuclear counterstaining with TO-PRO® 3. Bars a, b 10 μm; c 15 μm; d 4 μm; e 25 µm; f 8 µm