Fig. 1
Cobalt chloride induces barrier disruption in iPSC-derived BMECs but not in hCMEC/D3 monolayers. Cells were treated with cobalt chloride (CoCl2) for 24 h and were compared to untreated cell monolayers. Changes in the monolayer integrity were assessed by TEER (a) and by sodium fluorescein (b). Note the decrease in barrier function in IMR90-derived BMECs (iPSC-BMEC) versus the hCMEC/D3 monolayers. c Cobalt chloride induced decrease in adherent cell density in iPSC-BMEC at high concentrations (100 µM). * and ** denotes P < 0.05 and P < 0.01 versus control (untreated) groups. d Metabolic activity following treatment with CoCl2 did not induce cell toxicity in IMR90-derived BMECs, as we noted no decrease versus control group. Mean ± SD, n = 3 for each group, * and ** denotes P < 0.05 and P < 0.01 versus control (untreated) groups