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Fig. 5 | Fluids and Barriers of the CNS

Fig. 5

From: Fluid and ion transfer across the blood–brain and blood–cerebrospinal fluid barriers; a comparative account of mechanisms and roles

Fig. 5

Immunofluorescence staining of ion transporters in the choroid plexus of the IVth ventricle in a mouse or b rat. The Na+, K+-ATPase (red) is prominent in the apical brush border of the epithelial cells facing the lumen of the ventricle. The Na+, HCO3 cotransporter, NCBE/NBCn2 (green) is localized to the basolateral membranes of the epithelial cells facing the stroma (interstitium) in which are embedded the capillaries. Nuclei are stained with To-pro 3 DNA stain (blue). Scale bar 100 µm. Previously unpublished images provided by Dr. Jeppe Praetorius. Antibodies: Na+, K+-ATPase α1-subunit [537]; Slc4a10/Ncbe/NBCn2 [538] and To-pro 3 DNA stain (invitrogen). For a similar fluorescence image localizing NBCe2 to the brush border see [539]. For images that localize Na+, K+-ATPase to the brush border, AQP1 primarily but not exclusively to the brush border, and Ncbe/NBCn2 and AE2 to the basolateral membrane see [540, 541]

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