Fig. 2

Fluorescin-Aβ42 transit across an in vitro BBB model following ADAM10 inhibition. Fluorescin-Aβ42 (2 μM) was exposed to the basolateral (“brain”) side of the in vitro BBB model, while various concentrations of the ADAM10 inhibitor GI254023X (0, 0.1, 1, and 10 μM) were exposed to the apical (“blood”) compartment. Following incubation at 37 °C, samples were collected from the basolateral compartment at 60 min to assess the basolateral-to-apical transcytosis of fluorescin-Aβ42 across the BBB model. Values represent mean ± SEM (n = 3) and are expressed as the percentage change from control conditions. *p < 0.05; **p < 0.01; ***p < 0.001 as determined by one-way ANOVA followed by Tukey’s post hoc analysis