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Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: Directional cerebrospinal fluid movement between brain ventricles in larval zebrafish

Fig. 1

Experimental workflow. a Schematic representation of rhombencephalic ventricle Kaede injection and confocal photoconversion and imaging of zebrafish ventricles. b Workflow for image processing. Raw images were collected and then thresholded in Imaris (Bitplane) software so that red (photoconverted) Kaede was only detected after photoconversion and was not detected in the unconverted target ventricle immediately after photoconversion. Then the volume of the unconverted target ventricle was calculated over time by Imaris (Bitplane) software. Dashed line indicates telencephalic-to-mesencephalic/diencephalic boundary. c Workflow for data analysis. From volume measurements of the target ventricle, volumetric flow rate (Q) was calculated using Eq. (1) (see “Methods” section). Q was smoothed using a 9-point moving-window average. Q was then converted to linear velocity by dividing by the cross-sectional area of the smallest part of the aqueduct between the two ventricles. These CSF dynamics were reported as the maximum velocity between the two ventricles (vmax). T telencephalic ventricle, D/M diencephalic/mesencephalic ventricle, R rhombencephalic ventricle, hpf hours post-fertilization, V volume, Q volumetric flow rate, v velocity. Scale bar: 50 μm

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