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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: In vitro characterization of pralidoxime transport and acetylcholinesterase reactivation across MDCK cells and stem cell-derived human brain microvascular endothelial cells (BC1-hBMECs)

Fig. 2

a Schematic illustration of the modified transwell assay for measurement of coupled transcellular transport and enzymatic activity, and the chemical structure of 2-PAM. b Representative normalized absorbance versus time plots for reactivation of AChE. 1 AChE + ASCh: positive control (uninhibited enzyme + substrate). 2 AChE-OP + ASCh: negative control (inhibited enzyme + substrate). 3 AChE-OP + ASCh + 2-PAM: reactivation with no transport (inhibited enzyme + substrate + reactivator). 4 2-PAM//AChE-OP + ASCh: transcellular transport + reactivation. c Normalized AChE activity (dA/dt) obtained from absorbance versus time curves at the inflection point. The legend provides the details of each experiment. d Half-time for AChE reactivation. Data represent mean ± SD. All reactivation experiments were performed in HBSS

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