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Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: MicroRNA-155 contributes to shear-resistant leukocyte adhesion to human brain endothelium in vitro

Fig. 1

miR-155 modulates Jurkat and THP-1 firm adhesion to brain endothelial hCMEC/D3 cells. hCMEC/D3 cell monolayers were transfected with control scrambled Pre-miR and Pre-miR-155 (a, c, d) or control scrambled Anti-miR and Anti-miR-155 (a, c , e) followed by treatment with a combination of cytokines (TNFα + IFNγ) at 1 ng/ml for 24 h (a, d , e) or left unstimulated (a, b , c). a Representative images of shear-resistant firmly adhered Jurkat and THP-1 cells to hCMEC/D3 monolayer (field of view (FOV): 640 × 480 μm) used for quantification and b-e analysis of shear-resistant firmly adhered Jurkat and THP-1 cells to hCMEC/D3 expressed in number of cells/FOV. Experiments were carried out three to six times with five FOVs each. Data are mean ± SEM. Statistical analysis was performed using paired Student’s t test (*,# P < 0.05, **,## P < 0.01, #compared to unstimulated)

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