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Fig. 3 | Fluids and Barriers of the CNS

Fig. 3

From: Growth-factor reduced Matrigel source influences stem cell derived brain microvascular endothelial cell barrier properties

Fig. 3

Matrigel origin has a minor impact on BMECs drug efflux activity. a Drug uptake of rhodamine 123, BODIPY FL-prazosin and CM-DCFDA in presence of 5 μM cyclosporine A (CsA, P-gp inhibitor), 1 μM Ko143 (BCRP inhibitor) or 10 μM MK571 (pan-MRP inhibitor), respectively. Fluorescence values in controls (no inhibitor) were arbitrarily set to 100 %, * and ** denote P < 0.05 and P < 0.01 in comparison to B-Matrigel. b Doxorubicin accumulation profile in presence of CsA, Ko143 or MK571. Doxorubicin net uptake was calculated as previously described

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Fluids and Barriers of the CNS

ISSN: 2045-8118