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Fig. 6 | Fluids and Barriers of the CNS

Fig. 6

From: Modeling immune functions of the mouse blood–cerebrospinal fluid barrier in vitro: primary rather than immortalized mouse choroid plexus epithelial cells are suited to study immune cell migration across this brain barrier

Fig. 6

Barrier properties of titrated ECPC4 cells growing on Transwell filter inserts. a Different numbers of ECPC4 cells (5 × 104, 1 × 105, 5 × 105 and standard) were plated on the inverted porous filter membranes in triplicate. Standard = ECPC4 continuously split in a ratio 1:4, according to the distributer’s protocol (number of cells not counted). TEER was measured manually once per day on d3 and d4 in culture using the EndOhm device. The cells failed to build up a resistance irrespective of their seeding density. b The permeability coefficient (Pe) for Alexa Fluor 680–3 kDa dextran was assessed after the resistance measurement on d4. Bars represent the mean ± SD of all filters from two independent experiments, n = 6 for all groups

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