Fig. 6

Astrocyte extension and retraction across shunt catheter holes. Representative images of a single catheter hole in the short-chambered system taken between 16 and 51 days of culture. The sample was stained to reveal viable (green) and non-viable (red) cells. Viable astrocytes are observed in holes as early as 16 days after the perfusion system was initiated. The dynamic growth (and retraction) of cells is illustrated in the 37-, 44-, and 51-day images when cells were observed bridging across the hole or lining the catheter hole wall. These images are maximum projections of 3-D confocal images. Scale bar represents 100 µm