Table 1 Reagents/supplies and surgical tools required to perform the procedure
Surgical Tool | Company | Catalog No. |
|---|---|---|
Standard forceps, Dumont #5 | Fine Science Tools Inc., Foster City, CA, USA | 11251-30 |
Micro points, stainless steel, 15.5 cm, 45 degrees | Fine Science Tools | 10066-15 |
Double-pronged tissue pick, 11 cm, stainless steel | Fine Science Tools | 18067-11 |
Moria Bonn scissors, Inox, 9 cm, straight, sharp–sharp | Fine Science Tools | 14381-43 |
Hot glass-bead sterilizer | Fine Science Tools | 18000-45 |
Stereotaxic frame | Stoelting Co., Wood Dale, IL, USA | 51600 |
Gas anesthesia mask for mouse stereotactics | Harvard apparatus Inc, Holliston, MA, USA | 51609 M |
High-speed drill (Ideal Micro-Drill™ | CellPoint Scientific, Gaithersburg, MD, USA | 67-1200 |
Inhalational anesthesia vaporizer | EZ Anesthesia, Palmer, PA, USA | EZ-7000 |
Deltaphase® Isothermal Pad | Braintree Scientific Inc., Braintree, MA, USA | 39 DP |
Dissecting microscope | Carl Zeiss AG, Oberkochen, Germany | Stemi 2000 |
Reagents and Supplies | Company | Catalog no. |
Dexamethasone 2 mg/ml | Clipper Distributing Company, St Joseph, MO, USA | 18105-01 |
Isoflurane | Vedco Inc., St Joseph, MO, USA | NDC 50989-150-15 |
Alcohol prep pads | Thermo Fisher Scientific, Waltham, MA, USA | 22363750 |
PDI™ Povidone-Iodine Prep Pad | Thermo Fisher Scientific | 06-669-70 |
Saline solution (PBS) 0.9 % | Thermo Fisher Scientific | S5815 |
Artificial cerebral spinal fluid (ACSF) | Tocris Bioscience, Bristol, United Kingdom | 3525 |
Puralube® vet ophthalmic ointment | Dechra Pharmaceuticals, Northwich, United Kingdom | NA |
Super Glue LocTite® | LocTite® Brand–Consumer Products Henkel Corporation, Westlake, OH, USA | LOC1364076 |
Syringe, BD™ slip tip sterile | Thermo Fisher Scientific | 309659 |
Trimmer, Safe T-Light | Braintree Scientific | CLP-24 090 |
3 M™ Vetbond™ glue | Thermo Fisher Scientific | NC9604126 |