Fig. 6
From: Clearance from the mouse brain by convection of interstitial fluid towards the ventricular system
Role of the vasculature in bulk flow. a–c Cisterna magna injection, d–f striatum injection. Additional laminin staining is present in panels b–f. a Tracers were found in the subarachnoid space, embedding arteries (a) and veins (v). Arteries showed additional red autofluorescence from elastin. Green arrows F-500 followed paravascular spaces along arteries into the parenchyma. b F-500 was prominent in cisterns, where large arteries entered the brain (green arrow). c F-500 entered the paravascular space of vessels from the ventral side of the brain (green arrows). d Small vessels close to the injection site did not show tracers. e Choroid plexus with TR-3 and F-500, adjacent veins (v) did not show tracers. f Both tracers followed blood vessels towards the brain surface. F-500 accumulated at the pia mater. Scale bar 100 µm