Fig. 3

LAT1 mRNA decreased in vessels but not in neurons after LPS. A, B Darkfield emulsion autoradiography images show the distribution of LAT1 mRNA (silver grain accumulation, white) in the rat hypothalamus. Boxed areas from (A) are shown in higher magnification in (B). Low-level hybridization signal is distributed across the arcuate (Arc) and ventromedial nuclei (VMH), highly resembling a neuronal expression pattern; this signal does not change following LPS administration. Well-defined silver grains clusters over capillaries disappear 9 h after LPS, but become larger and more numerous at 48 h after LPS. 3v third ventricle, Arc, arcuate nucleus, ME median eminence, VMH ventromedial hypothalamic nucleus. Scale bar 200 µm on A; 100 µm on B. C LAT1 in situ hybridization signal in the hypothalamic paraventricular nucleus (PVH) of mice. While LAT1 signal in capillaries virtually disappears 9 h after LPS, the low-level neuronal LAT1 signal in the paraventricular nucleus remains visible. At 48 h after LPS, hybridization signal in vessels is markedly increased. 3v third ventricle, PVH hypothalamic paraventricular nucleus; Scale bar 100 µm