Table 1 Primer sequences used for RT-qPCR analysis
Primer | Reference sequence | Forward primer | Reverse primer |
|---|---|---|---|
Claudin-5 | NM_031701.2 | CTACAGGCTCTTGTGAGGACTTGAC | AGTAGGAACTGTTAGCGGCAGTTTG |
Occludin | NM_031329.2 | CTGACTATGCGGAAAGAGTCGACAG | AGAGGAATCTCCTGGGCTACTTCAG |
PECAM-1 | NM_031591.1 | ATTCTATAAGGACGATGCGCTGGTG | GCTGTTCAGTATCACGGTGCATTTG |
TfR 1 | NM_022712.1 | TGGATCAAGCCAGATCAGCATTCTC | TTTCTTCCTCATCTGCAGCCAGTTT |
ABCG2 | NM_181381.2 | GAGTTAGGCCTGGACAAAGTAGCAG | AAGAGGATGGAAGGGTCAGTGATGA |
ABCB1 | NM_012623.2 | AATCAACAGTACACAGACCGTCAGC | CCAAAGTGAAACCTGGATGTAGGCA |
HcRed1-C1 | pHcRed1-C1 (613-1377) | ATGTACATGGAGGGCACCGTGAA | GTCACGTGGATTCTCATGCTCTGG |
β-actin | NM_031144.3 | CCTCTGAACCCTAAGGCCAACCGTGAA | AGTGGTACGACCAGAGGCATACAGGG |