Hyperglycemic conditions, cigarette smoke extract and both combined affect expression of glucose transporters in membrane fractions of BBB endothelial cells. Sodium-independent and dependent glucose transporter proteins- GLUT-1 (a1) and SGLT-1 (a2) were significantly upregulated by CSE and HG + CSE in an additive pattern. GLUT-1 upregulation persisted at 24 h exposure (b1) however, SGLT-1 membrane expression returned to levels comparable to that of controls (b2). n = 3–4 biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control; $
p < 0.05 vs. HG and #
p < 0.05 vs. CSE.