Characterization of cultured brain microvascular cells from wild type (Wt) and ApoB-100 transgenic (Tg) mice. a Immunostaining of brain endothelial cells for transmembrane junctional protein claudin-5 (Cl-5) and occludin (Occl), and cytoplasmic linker proteins β-catenin (β-cat) and zonula occludens-1 (ZO-1). Astroglia cells were labeled for glial fibrillary acidic protein (GFAP), pericytes were stained for α-smooth muscle (α-SM) actin. Blue cell nuclei. Scale bar 15 µm. b Fluorescent intensity evaluation of immunostainings with ImageJ software shown as percentage of the labeling intensity of wild type cells. Values presented are mean ± SD, n = 8–16. Statistical analysis: two-way ANOVA followed by Bonferroni post-test, *p < 0.05, ***p < 0.001 wild type compared to transgenic group.