Figure 1
Immunohistochemical localization of EAAT1, EAAT2, EAAT3 and GS in neural cells around the rat lateral ventricle. Frozen sagittal sections from 6-week-old rats were stained with anti-EAAT1 (A), anti-EAAT2 (B), anti-EAAT3 (C), or anti-GS (D) antibodies (red). The nucleus was visualized by incubating with DAPI (blue). A, EAAT1 immunoreactivity (red) was observed in the cells lining the lateral ventricle (LV) (arrow heads, A 1 ), but not in the choroid plexus epithelial cells (CPx, arrows). At high magnification (A 2 -A 3 ), EAAT1 was localized on the apical membrane (arrow heads) of the ependymal cells (Ep). B, EAAT2 immunoreactivity (red) was not observed in the cells lining the LV (arrow heads, B 1 ) and choroid plexus epithelial cells (arrows, B 1 ) although it was detected in the parenchymal cells in the brain. At high magnification (B 2 -B 3 ), EAAT2 was not localized on the apical membrane (arrow heads) of the ependymal cells. C, EAAT3 immunoreactivity (red) was strongly detected in the choroid plexus epithelial cells (arrows) in the brain compared with the ependymal cells (arrow heads). At high magnification (C 2 -C 4 ), EAAT3 was localized on the apical membrane (arrows) of the choroid plexus epithelial cells. D, GS immunoreactivity (red) was observed in the cells lining the LV (arrow heads, D 1 ) as well as the parenchymal cells in the brain. At high magnification (D 2 -D 3 ), GS was expressed in the ependymal cells (arrow heads). Scale bar: 50 μm (A 1 , B 1 , C 1 , D 1 ), 2 μm (A 2 -A 3 , B 2 -B 3 , D 2 -D 3 ), 5 μm (C 2 -C 4 ).