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Figure 7 | Fluids and Barriers of the CNS

Figure 7

From: Hydrocortisone enhances the barrier properties of HBMEC/ciβ, a brain microvascular endothelial cell line, through mesenchymal-to-endothelial transition-like effects

Figure 7

Retardation of HBMEC/ciβ migration by hydrocortisone. Migration abilities of HBMEC/ciβ cultured with CSC-cbR, CSC-free, or CSC-HC were examined by scratch assay. The effect of RU486 on cell morphology was also tested using cells cultured with CSC-HC. Wound width was determined under microscopic observation at 0, 6 and 12 hours after the scratch had been made. The experiments were repeated three times and the representative results are shown. In the right side, the relative value of wound width was calculated as the value obtained at time 0 (immediately after scratch) was set to 1, and each value is expressed as mean ± S.D. obtained from three independent experiments. The asterisk indicates p < 0.05 compared to the value of CSC-free.

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