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Figure 6 | Fluids and Barriers of the CNS

Figure 6

From: Hydrocortisone enhances the barrier properties of HBMEC/ciβ, a brain microvascular endothelial cell line, through mesenchymal-to-endothelial transition-like effects

Figure 6

Promotion of endothelial cell differentiation of HBMEC/ciβ by hydrocortisone. (A) Cell morphology of HBMEC/ciβ cultured with CSC-cbR, CSC-free, or CSC-HC was analyzed by microscopy. The effect of RU486 on cell morphology was also tested using cells cultured with CSC-HC. The experiments were repeated four times and representative results are shown. (B) mRNA expression levels of representative endothelial cell differentiation marker genes (vWF and DARC) were determined by qPCR and calculated relative to the value obtained from CSC-cbR cells (set as basal level = 1). The “+” or “-” symbols indicate addition of RU486 or DMSO (0.1%) to the medium, respectively. Each value is expressed as mean ± S.D. obtained from three independent experiments. The single and double asterisks indicate p < 0.05 and p < 0.01, respectively, compared to CSC-free.

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Fluids and Barriers of the CNS

ISSN: 2045-8118