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Figure 3 | Fluids and Barriers of the CNS

Figure 3

From: Hydrocortisone enhances the barrier properties of HBMEC/ciβ, a brain microvascular endothelial cell line, through mesenchymal-to-endothelial transition-like effects

Figure 3

Involvement of glucocorticoid receptor in the effects of hydrocortisone on HBMEC/ciβ barrier function. (A) GR protein expression was examined by Western blot analysis. The arrowhead indicates 100 kDa. (B) mRNA expression level of representative GR-target genes (ANXA1, GILZ, and NFκBIA) in cells cultured with CSC-cbR, CSC-free, or CSC-HC were examined by qPCR. Effect of a GR antagonist, RU486 (RU), on HC-mediated induction of mRNA expression was also investigated. mRNA expression levels were calculated relative to the value obtained from CSC-cbR cells (set as the basal level =1). (C) Effects of RU486 on HC-mediated barrier reinforcement was examined by determining Na-F permeability (left) and TEER (right). Each value in the above experiments is expressed as mean ± S.D. obtained from three independent experiments. In (B) and (C), the “-” symbol indicates DMSO (0.1%) was added to the medium as a control. The single and double asterisks indicate p < 0.05 and p < 0.01, respectively, compared to the value of CSC-free in (B) or CSC-cbR in (C).

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Fluids and Barriers of the CNS

ISSN: 2045-8118