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Figure 2 | Fluids and Barriers of the CNS

Figure 2

From: Hydrocortisone enhances the barrier properties of HBMEC/ciβ, a brain microvascular endothelial cell line, through mesenchymal-to-endothelial transition-like effects

Figure 2

Identification and characterization of barrier strengthening effects of hydrocortisone on HBMEC/ciβ-based blood–brain barrier model. (A) The effect of cbR withdrawal from the culture medium on HBMEC/ciβ barrier properties was examined. The “-” symbol indicates the absence of cbR. (B) SQs components were screened for identification of the factors responsible for barrier property improvement. Here, the “-” symbol indicates the absence of growth factors. (C) The effect of cbR on the HC-mediated decrease in Na-F permeability of HBMEC/ciβ was examined. Here, the “-” symbol indicates the absence of HC. (D) The effect of HC on barrier function of HBMEC/ciβ was confirmed by determining the relative Pe values of Na-F (left) and sucrose (right) permeability. The value obtained from the cells cultured with CSC-HC was calculated relative to the value obtained from CSC-cbR cells (set as basal level =1) in each assay. (E) TEER was examined and compared between HBMEC/ciβ cultured with CSC-cbR and CSC-HC. The values of Na-F permeability (Papp or Pe) (×10−3 cm/min) and TEER (Ω × cm2) were expressed as mean ± S.D. of three independent experiments. The single, double, and triple asterisks indicate p < 0.05, p < 0.01, and p < 0.005, respectively, compared to the value of CSC-SQs in (B) or CSC-cbR in (D) and (E).

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