Figure 1
From: Expression and localization of claudins-3 and -12 in transformed human brain endothelium
Claudin-3 and Claudin-12 expression in hCMEC/D3 cells. A: Immunoblots showing claudin-3 (Cld 3, upper panel) and claudin-12 (Cld 12, lower panel) expression in whole cell lysates derived from primary human brain (lane 1, PHB) and hCMEC/D3 endothelial cells (lanes -2, D3 p31 and -3, D3 p26) in addition to glioblastoma cells (lane 4, U87) or cells of fibroblast origin (lanes-6, MEF1; -7, Pea-13; -8, NIH 3T3), epithelial (lanes -5, MCF-7 and -9, MDCK) origin. B-E: hCMEC/D3 cells (B, D) grown to confluence and further rested for 48 h or confluent cultures of MDCK cells (C, E) were stained with antibodies to claudin-3 (B, C) or claudin-12 (D, E). Isotype matched antibodies did not stain cells (data not shown). Scale bar = 25 μm.