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Figure 4 | Fluids and Barriers of the CNS

Figure 4

From: Detection of oligoclonal IgG kappa and IgG lambda bands in cerebrospinal fluid and serum with Hevylite™ antibodies. comparison with the free light chain oligoclonal pattern

Figure 4

Case report (case 11 in Table 1): CSF (C)and serum (S) findings. 4a. Lymphocytic pleocytosis and 2 plasma cells in the CSF cytospin preparation. 4b. Isoelectric focusing/immunofixation using a commercial kit on Hydrasys instrument (Sebia) revealed CSF-restricted oligoclonal IgG bands as well as identical paraprotein bands in CSF and serum. Anode is on the left. 4c. Serum immunofixation electrophoresis (Sebia) revealed the presence of IgGκ monoclonal band (arrow). Anode is at the top. ELP, electrophoresis (general protein stain); G, A, M, K, L: immunofixation for γ, α, μ, κ and λ chain, respectively. 4 d. Isoelectric focusing/affinity-mediated immunoblotting analysis of case 11: Paraprotein bands did not react with Borrelia antigen but partly obscured CSF-restricted oligoclonal IgG pattern. Paired CSF and serum samples tested for (from left to right) "total" IgG, IgGκ, IgGλ; Anti-Borrelia IgG, IgGκ, IgGλ. Anode is at the top. Samples were analyzed on the same gel using a mixture of 6.2% Pharmalyte pH 3-10 and 1.7% Pharmalyte pH 8-10.5.

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Fluids and Barriers of the CNS

ISSN: 2045-8118