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Figure 2 | Fluids and Barriers of the CNS

Figure 2

From: Detection of oligoclonal IgG kappa and IgG lambda bands in cerebrospinal fluid and serum with Hevylite™ antibodies. comparison with the free light chain oligoclonal pattern

Figure 2

Isoelectric focusing/affinity-mediated immunoblotting analysis of IgG (a), IgGκ (b), IgGλ (c), free κ (d), - and free λ (e) OCBs in 6 paired CSF (C) and serum (S), C 1 -C 6 , S 1 - S 6 , cases 9, 33, 29, 30, 31, and 10 (Table 1). CSF 1 and CSF 6 were positive in all tests; however, the majority of IgG bands were of the lambda type in CSF 1, whereas of the kappa type in CSF 6 (arrowheads). Numerous free κ and free λ bands were present in CSF 1, whereas more free κ than free λ bands were present in CSF 6. Sample 2 is IgGλ paraprotein clearly detected using Hevylite IgGλ with no cross-reaction with IgGκ. Sample 3 represents "type 4", i.e. bands of equal intensity in CSF and serum that are both of IgGκ and IgGλ type (arrowheads). No intrathecal synthesis of IgG and no free light chain bands were detected. Finally, samples 4 and 5 represent normal (negative) results with no clear oligoclonal bands. Anode is at the top. A mixture of 6.2% Pharmalyte pH 3-10 and 1.7% Pharmalyte pH 8-10.5 was used for IgG, IgGκ, and IgGλ, whereas 6.4% Pharmalyte was only used for free κ and free λ.

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