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Figure 1 | Fluids and Barriers of the CNS

Figure 1

From: Detection of oligoclonal IgG kappa and IgG lambda bands in cerebrospinal fluid and serum with Hevylite™ antibodies. comparison with the free light chain oligoclonal pattern

Figure 1

Affinity immunoblots to estimate the detection limit of the IEF/AIB using several IgG paraproteins. From left to right: total IgG, IgGκ, IgGλ. Based on serum protein electrophoresis and densitometry, serum IgGκ paraprotein (Sκ) represented 67.6% of total IgG, and serum IgGλ paraprotein (Sλ) represented 18.6% of total IgG. Using Hevylite antibodies, Sκ is clearly visible at an IgG concentration 0.05 mg/l, i.e. 0.3 ng of IgG or 0.2 ng of the paraprotein; Sλ is clearly visible at an IgG concentration 0.2 mg/l, i.e. 1.2 ng of IgG or 0.2 ng of the paraprotein (arrows). In total IgG OCB assay, the detection limit is several times higher but still very good (not higher than 1 ng of paraprotein). The percentage of paraprotein from total IgG was not known for paired CSF (C) and serum (S') sample (UK NEQAS Control 8/2011). The anode is at the top. A mixture of 6.2% Pharmalyte pH 3-10 and 1.7% Pharmalyte pH 8-10.5 was used for isoelectric focusing.

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Fluids and Barriers of the CNS

ISSN: 2045-8118