Figure 5
Ecrg4 over-expression inhibits injury-induced proliferation of subependymal progenitor cells. A: Transduction of ependyma with adenovirus vector containing the green fluorescent protein gene (AdGFP). If Ad GFP was injected i.c.v. such that target cells were labeled prior to the penetrating injury, ependymal cells throughout the ventricles were transduced as indicated by the GFP staining of ependyma (green). Scale bar = 100 μm. B: Cell proliferation after penetrating injury after AdGFP. To monitor cell growth after injury, animals that had been injected with AdGFP, received daily injections of BrdU i.p. as described in the text and the incorporation of BrdU into DNA was evaluated by immunohistochemistry. Brdu: red, DAPI: blue, scale bar = 20 μm. C: Cell proliferation after penetrating injury and AdEcrg4. If instead of AdGFP as above, the target ependymal cells were transduced by i.c.v. injection of adenovirus containing Ecrg4 (Ad Ecrg4 ), there was a decrease in BrdU-labeled cells in the subependymal zone. Scale bar = 20 μm. D: Quantification of cell proliferation after penetrating injury after AdGFP or AdEcrg4. When apparent differences in proliferating cell number were quantified, a significant difference in proliferating cell number was observed (p < 0.01). Error bars represent mean ± standard deviation (n = 4).