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Figure 2 | Fluids and Barriers of the CNS

Figure 2

From: Ecrg4 expression and its product augurin in the choroid plexus: impact on fetal brain development, cerebrospinal fluid homeostasis and neuroprogenitor cell response to CNS injury

Figure 2

The choroid plexus and ependyma are major sites of Ecrg4 expression. A: Ecrg4 gene expression in the mouse embryo. In situ hybridization of mouse embryo day E14.5 were obtained from the GenePaint consortium [51]. Significantly more data is available at: http://www.genepaint.org/cgi-bin/mgrqcgi94?APPNAME=genepaint&PRGNAME=analysis_viewer&ARGUMENTS=-AQ64622747128404,-AEG,-A804,-Asetstart,-A5 B: Ecrg4 gene expression in the developing mouse brain. Higher magnification analysis of the CNS localization (from samples shown in A), shows the in situ hybridization signal localizing primarily to choroid plexus and ependyma. C: Ecrg4 gene expression in the adult mouse brain. In situ hybridization of adult mouse brain were obtained from open source data generated by the Allen Brain Atlas [21]. Significantly more data throughout the brain are available for analyses at: http://mouse.brain-map.org/brain/1500015O10Rik/70429477/thumbnails.html?ispopup=1. D: Ecrg4 gene expression in the mouse choroid plexus and ependyma. A higher magnification analysis of the CNS localization (from samples shown in Panel C), show that the in situ hybridization signal localizes primarily to choroid plexus epithelial cells and to a lesser extent ventricular ependymal cells. E: Ecrg4 gene expression in mouse central canal ependymal/epithelial cells in the spinal cord. In situ hybridization maps of adult mouse spinal cord were selected from open source data generated by the Allen Brain Atlas Consortium [21]. Significantly more data on the distribution of Ecrg4 (Riken150001O15) gene expression throughout the mouse spinal cord, including other cell types, is available at http://mousespinal.brain-map.org/imageseries/show.html?id=100028769. F: Quantitative gene expression by RT-qPCR. After extracting tissues from donor adult mice (n = 12), highest Ecrg4 expression was found in the dissected choroid plexus. Data was calculated using the ∆ΔCt method and normalized to the values obtained in testes. Similar findings were obtained in a survey of male rats (not shown) and low gene expression was detectable in all tissues. Error bars represent mean ± standard deviation, n = 5.

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Fluids and Barriers of the CNS

ISSN: 2045-8118