Attenuation of prostaglandin E2 elimination across the mouse blood-brain barrier in lipopolysaccharide-induced inflammation and additive inhibitory effect of cefmetazole

Table 3 Effect of intracerebral pre-administration of antibiotics and NSAIDs (5 mM) on [³H]PGE₂ elimination from brain across the BBB in LPS-treated mice.

Compound	Percentage of [³H]PGE₂ remaining in ipsilateral cerebrum (100-BEI, %)	Percentage of control
*Cephalosporins*
Control	91.9 ± 6.6	100
Cefmetazole	133 ± 16*	146
Cefotaxime	136 ± 1*	149
Ceftriaxone	113 ± 11	124
Cefsulodin	94.2 ± 15.3	103
Cefazolin	85.7 ± 6.2	94.0
*Other antibiotics and NSAIDs*
Control (0.25% DMSO)	97.2 ± 11.1	100
Indomethacin (0.25% DMSO)	114 ± 9	117
Ketoprofen (0.25% DMSO)	82.8 ± 5.8	85.2
Clarithromycin (0.25% DMSO)	82.2 ± 16.4	84.6

Each compound or ECF buffer with or without 0.25% DMSO (control) was administered at 5 min prior to administration of [³H]PGE₂ in the brain of LPS-treated mice. The percentage of [³H]PGE₂ in the ipsilateral cerebrum was determined at 40 min after intracerebral [³H]PGE₂ microinjection. Each value represents the mean ± S.E.M. (n = 3-8). *p < 0.05, significantly different from control.

ISSN: 2045-8118