Figure 6
Effect of DEP exposure on Cyp1b1 expression in isolated rat brain microvessels. Western blots showing Cyp1b1 protein expression; β-actin was used as loading control. (A) Ex vivo DEP exposure of brain microvessels increased Cyp1b1 in a concentration-dependent manner. (B) Carbon black particles were used as a negative control for particulate matter and did not have any effect on Cyp1b1 protein levels in brain microvessels. (C) Cyp1b1 protein expression was increased in brain microvessels from rats that were exposed in vivo to 0.5 and 2 mg/m3 diesel engine exhaust containing DEP for 5 h/day, 5 days/week for 4 consecutive weeks. Brain capillaries were pooled from 6 rats per treatment group.