Figure 2
Effect of 1,25(OH) 2 D3 on amount of [125I]hAβ(1-40) remaining in the ipsilateral cerebrum. (A) Dose-dependence of the effect of 1,25(OH)2D3 on [125I]hAβ(1-40) elimination from mouse brain. Mice received i.p. injection of 1,25(OH)2D3 (0.2, 1 or 5 μg dissolved in 100 μL of H2O containing 5% ethanol) or vehicle (V, 100 μL of H2O containing 5% ethanol). After 24 h, a mixture of [125I]hAβ(1-40) (0.012 μCi) and [3H]dextran (0.12 μCi) dissolved in 0.30 μL of ECF buffer was injected into the S2 region of the brain. Data, obtained at 60 min after intracerebral microinjection, are presented as the mean ± SEM (n = 5). (B) Time-course of the effect of i.p. administration of 1,25(OH)2D3 on [125I]hAβ(1-40) elimination from mouse brain. Mice received i.p. injection of 1,25(OH)2D3 (D3, 1 μg dissolved in 100 μL of H2O containing 5% ethanol) or vehicle (V, 100 μL of H2O containing 5% ethanol). After 24, 48 and 72 h, a mixture of [125I]hAβ(1-40) (0.012 μCi) and [3H]dextran (0.12 μCi) dissolved in 0.30 μL of ECF buffer was injected into the S2 region of the brain. Data, obtained at 60 min after intracerebral microinjection, are presented as the mean ± SEM (n = 4 - 5). *p < 0.05, **p < 0.01, significantly different from vehicle-treated group.