Figure 6

Altered glycaemia-induced increase in BBB endothelial cell inflammation. Following exposure to normal or altered glycemic conditions for 3 h (A) and 24 h (B), hCMEC/D3 cells were co-stained for the expression and distribution of VE-cadherin (red) and VCAM (green), important modulators of inflammation. Images were captured at 40× (scale: 100 μm) and merged with DAPI. Representative western blots were shown with β-tubulin as loading control. Data were expressed as mean ± S.E.M. (% control). N = 3-4 samples/condition and replicated twice.