Effects of hyper and hypoglycemia on the expression and distribution of glucose transporters. Following exposure to control or altered glycemic conditions, hCMEC/D3 cells were co-stained for immunofluorescence analysis of GLUT-1 (green) and SGLT-1 (red) expression and distribution at 3 h (A) and 24 h (B). Images were captured at 40× (scale: 100 μm) and merged with DAPI. Results were corroborated by western blot analyses at 3 and 24 h. Expression of GLUT-4 was assessed by western blot analysis at 24 h following altered glycemia (C). Data were expressed as mean ± S.E.M. Representative western blots were shown with β-tubulin as loading control. *** p < 0.001, ** p < 0.01, * p < 0.05 and # p < 0.05, vs. control. N = 3-4 samples/condition and replicated twice.