Figure 2

Effects of altered glycaemia on expression and distribution of TJ proteins, ZO-1 and claudin-5. Following exposure to control, hypo or hyperglycemia, hCMEC/D3 cells were dual-labeled for ZO-1 (green) localization by immunofluorescence analysis at 3 h (A) and 24 h (B). Images were captured at 40× (scale: 100 μm) and merged with DAPI. Immunofluorescence images were paired with corresponding western blot analyses at 3 and 24 h. Data were expressed as mean ± S.E.M. Representative western blots were shown with β-tubulin as loading control. *** p < 0.001, ** p < 0.01, * p < 0.05 vs. control. N = 3-4 samples/condition and replicated twice.