Figure 1

Effect of sleep fragmentation (SF) on EAE scores and disease progression. SF was applied from 10 days before EAE induction until 16 days after induction. (A) Three groups of mice (n = 8 /group) were studied: without induction of EAE (naïve), induction of EAE with 100 μg /mouse of MOG_35–55 without SF (resting) and with SF (ZT 0–12 SF). By the end of the study on day 16, there was no difference in EAE score between the resting EAE group and the SF EAE group (ZT0-12 SF). (B) Two groups of mice (n = 8/group) with EAE induction with 80 μg/mouse of MOG_35–55 were monitored until day 28. In the SF EAE group (ZT 0–12 SF), the EAE scores were lower in the recovery phase (Day 16–28) than resting EAE, shown both by the daily progression and the cumulative score (inset). *: p < 0.05.