Skip to main content

Table 2 Results of antioxidant treatment on hydrocephalic rats (Trial 2)

From: Oral antioxidant therapy for juvenile rats with kaolin-induced hydrocephalus

 

Nonhydrocephalic controls

Vehicle (30% canola)

Dextrose

Low dose antiox.†

High dose antiox.†

Sample size

8

11

10

11

10

Ventricle area index (pre-treat)

0.006 ± 0.0007

0.114 ± 0.013**

0.119 ± 0.016**

0.107 ± 0.015**

0.118 ± 0.015**

Ventricle area index (post-treat)

0.012 ± 0.003

0.140 ± 0.018**#

0.169 ± 0.027**#

0.125 ± 0.013**#

0.140 ± 0.010**#

Percent enlargement ventricles during treatment

-

22.5 ± 8.1

40.1 ± 6.2

23.0 ± 6.8

25.1 ± 8.3

Body weight (g) (pre-treat)

169.3 ± 4.6

123.3 ± 7.2**

135.2 ± 8.2**

116.9 ± 6.4**

112.7 ± 5.3**

Body weight (g) (post-treat)

269.3 ± 6.7#

200.2 ± 13.4**#

219.4 ± 13.5*#

175.4 ± 12.5**#

179.6 ± 12.0**#

Rearing activity (post-treat) (beam breaks - 5 min)

100 ± 12

67 ± 12

96 ± 8

78 ± 16

82 ± 16

Ambulatory activity (post-treat) (beam breaks - 5 min)

466 ± 35

334 ± 31*#

548 ± 54#

461 ± 39#

396 ± 53#

Total activity (post-treat) (beam breaks - 5 min)

618 ± 41

459 ± 38*#

713 ± 54#

637 ± 41#

558 ± 53#

Endurance of rotarod (cont. speed, post-treat) (sec)

33 ± 8

32 ± 10

37 ± 7

66 ± 15#

38 ± 11

Endurance of rotarod (accelerating speed, post-treat) (sec)

57 ± 15

56 ± 11#

73 ± 14#

65 ± 13#

71 ± 13#

Morris water maze test (post-treat, 3 trials mean) (sec)

7.67 ± 0.85#

6.98 ± 1.10#

5.89 ± 0.61#

5.15 ± 0.41*#

4.46 ± 0.46**#

Swim time 150 cm (post-treat) (sec)

24.3 ± 6.7

13.4 ± 2.0

20.9 ± 3.4

18.0 ± 3.4#

17.0 ± 3.1

Lateral corpus callosum thickness (μm)

315 ± 16

208 ± 14**

220 ± 20**

220 ± 18**

208 ± 13**

GFAP content (ELISA) frontal cerebrum (mg GFAP/g protein)

0.68 ± 0.16

1.08 ± 0.19

1.29 ± 0.24

1.11 ± 0.16

1.10 ± 0.25

MBP content (ELISA) frontal cerebrum (mg MBP/g protein)

2.30 ± 0.26

2.42 ± 0.28

2.91 ± 0.28

2.70 ± 0.35

2.30 ± 0.16

MDA level (TBARS) parietal cerebrum (μM MDA/g wet tissue)

111.98 ± 10.57

145.32 ± 8.94*

138.99 ± 9.41

146.89 ± 9.67*

141.35 ± 8.45*

ABTS + content (antioxidant capacity) hippocampus (mM)

0.580 ± 0.029

0.582 ± 0.025

0.467 ± 0.062

0.589 ± 0.019

0.574 ± 0.026

  1. All data are presented as mean ± SEM.
  2. †The low and high dose antioxidant combinations were made up in 0.45% saline with 30% canola oil.
  3. *p < 0.05 control rats vs. hydrocephalic groups, Student’s t-tests or ANOVA with Bonferroni-Dunn post hoc tests for intergroup comparisons.
  4. **p < 0.005 control rats vs. hydrocephalic groups, Student’s t-tests or ANOVA with Bonferroni-Dunn post hoc tests for intergroup comparisons.
  5. #p < 0.05 before (pre-treat) vs. after (post-treat) treatment, Student’s t-tests.