Figure 2

EB labels striatal neurons upon GA perinatal administration. (A) Confocal images of the striatum evidencing that in GA-injected animals EB leaked into the parenchyma and entered many cells that surrounded the blood vessel (white dashed lines) and have a typical neuronal morphology (white arrows). In contrast, in vehicle-injected animals EB was restricted to the blood vessel wall. (B) EB did not permeate the cell membrane of S100β positive astrocytes in GA-injected animals (green, short white arrows) either in the striatal parenchyma or in the processes close to blood vessels, but it stained numerous rounded cells (long white arrows) that resembled the morphology of striatal neurons. (C) Lectin-positive microglia (arrowheads) were not labeled with EB. It was also evident that microglia are in close contact with EB positive neuron-like (white arrow) cells in GA-injected animals. (D) Co-localization of EB (red) and NeuN (green) was observed in many striatal neurons (yellow, white arrows, lower picture) in GA-injected animals. In contrast, the striatum from vehicle-injected animals did not show co-localization and green NeuN positive cells were typically arranged (upper picture). The quantitation of double labeled EB/NeuN neurons evidences a significant increase (* at p < 0.05) both at 14 and 30 DPI, with respect to controls. Scale bars in A and C: 15 μm B and D: 50 μm.