Removal of albumin and immunoglobulins from canine cerebrospinal fluid using depletion kits: a feasibility study

Günther, Ramona; Krause, Eberhard; Schümann, Michael; Ausseil, Jérome; Heard, Jean-Michel; Blasig, Ingolf E; Haseloff, Reiner F

doi:10.1186/2045-8118-11-14

Fluids and Barriers of the CNS

Table 1 Proteins identified by mass spectrometry with exponentially modified protein abundance index (emPAI) > 1.0 ¹

From: Removal of albumin and immunoglobulins from canine cerebrospinal fluid using depletion kits: a feasibility study

Protein	Accession No.²	M.W.³	emPAI_max⁴
ProteoSeek Depletion Kit
Serum albumin precursor [Canis lupus familiaris]	gi\|55742764	69	11.85
Hemoglobin subunit beta-like [Canis lupus familiaris]	gi\|399567834	16	6.30
PREDICTED: Serum albumin isoform X1 [Canis lupus familiaris]	gi\|545520919	69	4,41
PREDICTED: Tetranectin [Canis lupus familiaris]	gi\|545535577	18	4.28
PREDICTED: Apolipoprotein A-I [Canis lupus familiaris]	gi\|73955106	30	3.34
PREDICTED: apolipoprotein E isoform X5 [Canis lupus familiaris]	gi\|545488191	47	3.20
Immunoglobulin heavy chain variable region [Mus musculus]	gi\|81237613	13	2.90
PREDICTED: Complement component C7 isoform 1 [Canis lupus familiaris]	gi\|73953824	95	2.21
PREDICTED: Immunoglobulin lambda-like polypeptide 5-like isoform X1 [Canis lupus familiaris]	gi\|545544681	25	2.12
Prostaglandin-H2 D-isomerase precursor [Canis lupus familiaris]	gi\|50978842	21	2.08
Hemoglobin subunit alpha [Canis lupus familiaris]	gi\|44888810	15	1.96
PREDICTED: Immunoglobulin lambda-like polypeptide 5-like isoform X2 [Canis lupus familiaris]	gi\|545544683	25	1.77
PREDICTED: Gelsolin [Canis lupus familiaris]	gi\|545518174	94	1.70
PREDICTED: Fibulin-1 isoform X1 [Canis lupus familiaris]	gi\|545514459	78	1.63
Immunoglobulin gamma heavy chain A [Canis lupus familiaris]	gi\|17066524	52	1.63
Immunoglobulin gamma heavy chain B [Canis lupus familiaris]	gi\|17066526	52	1.61
PREDICTED: EGF containing fibulin-like extracellular matrix protein 1 isoform X3 [Canis lupus familiaris]	gi\|57092953	55	1.60
PREDICTED: Angiotensinogen [Canis lupus familiaris]	gi\|545494757	52	1.53
Immunoglobulin heavy chain constant region CH2 [Canis lupus familiaris]	gi\|124390009	12	1.45
Ig heavy chain V region GOM [Canis lupus familiaris]	gi\|123768	12	1.37
Immunoglobulin heavy chain constant region CH4 [Canis lupus familiaris]	gi\|124390013	14	1.21
PREDICTED: LOW QUALITY PROTEIN: Serotransferrin isoform 1 [Canis lupus familiaris]	gi\|545539001	85	1.16
Protease serine 4 isoform B [Homo sapiens]	gi\|33126535	28	1.13
Clusterin precursor [Canis lupus familiaris]	gi\|50979240	52	1.08
PREDICTED: Plasminogen isoform X1 [Canis lupus familiaris]	gi\|545485785	91	1.06
Vivapure Depletion Kit
Serum albumin precursor [Canis lupus familiaris]	gi\|55742764	69	11.30
Hemoglobin subunit beta-like [Canis lupus familiaris]	gi\|399567834	16	5.69
PREDICTED: Apolipoprotein A-I [Canis lupus familiaris]	gi\|73955106	30	4.80
PREDICTED: Angiotensinogen [Canis lupus familiaris]	gi\|545494757	52	3.35
PREDICTED: Serum albumin isoform X1 [Canis lupus familiaris]	gi\|545520919	69	2.98
Hemoglobin subunit alpha [Canis lupus familiaris]	gi\|44888810	15	2.91
Immunoglobulin gamma heavy chain B [Canis lupus familiaris]	gi\|17066526	52	2.58
PREDICTED: Immunoglobulin lambda-like polypeptide 5-like isoform X1 [Canis lupus familiaris]	gi\|545544681	25	1.62
PREDICTED: Immunoglobulin lambda-like polypeptide 5-like isoform X2 [Canis lupus familiaris]	gi\|545544683	25	1.62
Prostaglandin-H2 D-isomerase precursor [Canis lupus familiaris]	gi\|50978842	21	1.33
Albumin, isoform CRA_h [Homo sapiens]	gi\|119626071	69	1.21
Immunoglobulin heavy chain constant region CH2 [Canis lupus familiaris]	gi\|124390009	12	1.18
Immunoglobulin gamma heavy chain A [Canis lupus familiaris]	gi\|17066524	52	1.16
beta-2-Glycoprotein 1 precursor [Canis lupus familiaris]	gi\|54792721	38	1.00
ProteoExtract Depletion Kit
Immunoglobulin heavy chain variable region [Mus musculus]	gi\|81237613	13	2.39
Hemoglobin subunit alpha [Canis lupus familiaris]	gi\|122508	15	1.64

¹The complete list of identified proteins is available at Additional file 2.
²Accession number in NCBI database.
³M.W., Protein molecular weight in kDa.
⁴The emPAI value expresses mass spectrometry-based quantitation of protein abundance and is calculated according to $emPAI = 10^{\frac{N_{observed}}{N_{observable}}} - 1$ , (N_observed, N_observable, number of observed/observable peptides); cf. http://www.matrixscience.com/help/quant_empai_help.html for more details.

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ISSN: 2045-8118

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