Table 1 Sense and antisense primers for quantitative PCR
Target mRNA | Sequence(5’-3’) | Product size |
|---|---|---|
(Accession number) | (bp) | |
hOCT1/SLC22A1 | AATGGACCACATCGCTCAAAA | |
(NM_003057) | CTTCGAGGGAAAGCATCTTTAAAT | 68 |
hOCT2/SLC22A2 | GGACGGCTGGGTGTACGA | |
(NM_003058) | GGAGTTGGCACATACCAGGTTAA | 70 |
hOCT3/SLC22A3 | CATGCCTTGTCACTGCGTTCT | |
(NM_021977) | ATGTAGCCACTGTGGTCCTCAA | 63 |
hOCTN1/SLC22A4 | CCAGAGTGGGCAGCATCAT | |
(NM_003059) | TAGGGCAGCATTCTGTTGTAAGC | 67 |
hOCTN2/SLC22A5 | CCTTCTCTTCATGCAGCTGGTA | |
(NM_003060) | CCCACCATCACCAGGACTGT | 66 |
hMATE1/SLC44A1 | CAGTCACGCTGGCAATCG | |
(NM_080546) | GGTGTCACAGGCAGAAGATAAGC | 77 |
hMATE2/SLC44A2 | TCTGAGAGGAACTGGGAAGCA | |
(NM_152908) | AGGCCGATGATGTAATATGTGATG | 69 |
hPMAT/SLC29A4 | CAGCTTTCACGGATACTACATTGG | |
(NM_001040661) | GCAAAGTAGATGGCGTGATAACG | 67 |
hGAPDH | CCACATCGCTCAGACACCAT | |
(NM_002046) | GCGCCCAATACGACCAAAT | 66 |