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Figure 2 | Fluids and Barriers of the CNS

Figure 2

From: Functional expression of a proton-coupled organic cation (H+/OC) antiporter in human brain capillary endothelial cell line hCMEC/D3, a human blood–brain barrier model

Figure 2

[3H]pyrilamine uptake by hCMEC/D3 cells. (A) Time course of the cell-to-medium (C/M) ratio of [3H]pyrilamine uptake by hCMEC/D3 cells. Uptake of [3H]pyrilamine (74 kBq/μL, 90 nM) was measured at 37°C. (B) Concentration-dependence of the initial uptake rate of [3H]pyrilamine in hCMEC/D3 cells. hCMEC/D3 cells were preincubated with incubation buffer (122 mM NaCl, 3 mM KCl, 25 mM NaHCO3, 1.2 mM MgSO4, 1.4 mM CaCl2, 10 mM D-glucose, 10 mM HEPES, pH 7.4) for 20 min at 37°C. Uptake of [3H]pyrilamine (74 kBq/μL, 90 nM) was measured at 37°C for 10 sec. (C) Eadie-Hofstee plot of [3H]pyrilamine uptake by hCMEC/D3 cells. V, initial uptake rate (nmol/mg protein/10 sec). [S], [3H]pyrilamine concentration in the transport medium (μM). Each point represents the mean ± S.E. from three to four determinations. When verticals are not shown, the S.E. is contained within the limits of the symbols.

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