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Table 1 Parameters analyzed by intravital microscopy (modified from[22])

From: Live cell imaging techniques to study T cell trafficking across the blood-brain barrier in vitro and in vivo

Parameter

Unit

Formula

Total Cellular Flux (TFx)

min-1

Number of T cells that pass through a vessel during a given observation period

Initial Contact Flux (ICFx)

min-1

Number of immune cells that interact, either by rolling or getting captured to, with the vessel wall during an observation period

Initial Contact Fraction (ICF)

%

ICF = ICFx/TFx × 100

Rolling Flux (RFx)

min-1

Number of immune cells that roll along the vessel wall during an observation period

Rolling Fraction (RF)

%

RF = RFx/TFx × 100

Capture Flux (CFx)

min-1

Number of immune cells that are captured to the vessel wall during an observation period

Capture Fraction (CF)

%

CF = CFx/TFx × 100

Firm Adhesion/Field Of View (FOV)

none

Number of firmly adherent T cells per one field of view (FOV) within the spinal cord window