Figure 5

Quantification of OT-I CD8⁺T cell firm adhesion to the post capillary venules of the spinal cord microvasculature of C57BL/6 mice during EAE. Permanently adhering OT-I T cells were counted 10 min, 30 min and 1 hour after cell infusion. Each dot represents the number of adherent OT-I T cells/field of view (FOV). The numbers of mice analyzed at t = 10 min for each condition was n = 8 for rat IgG2b, n = 6 for anti-α4β7, n = 6 for anti-β7 and n = 8 for anti-α4. At t = 30, n = 8 for rat IgG2b, n = 6 for anti-α4β7, n = 6 for anti-β7 and n = 4 for anti-α4. At time t = 1 h, the number of mice was n = 7 for rat IgG2b, n = 5 for anti-α4β7, n = 5 mice for anti-β7 and n = 5 for anti-α4. Data are presented as mean values +/− standard deviation (SD). Mann–Whitney U-Test was used for comparisons between different data sets. Asterisks indicate significant differences (*P < 0.05, and ***P < 0.005), n.s.: not significant.