Figure 2

The migratory phenotype of T cells. Representative experiments of T cell interactions with TNF-α stimulated pMBMECs in vitro under flow conditions during a period of 15 minutes (2A) or for 3 different time periods of 10, 15 or 20 minutes (2B). The behavior of each arrested T cell was analyzed by eye in an offline analysis of the time-lapse videos and assigned to one category and expressed in percentage of initially arrested T cells. Arrested T cells that crawled into or out of the FOV during the recording time were excluded from the analysis. “Crawling”: T cells that polarized and crawled at least two T cell diameter distance but did not diapedese across the endothelium. “Crawling/partial diapedesis”: T cells that polarized, crawled and started but did not complete diapedesis during the indicated time period. “Crawling/Diapedesis”: T cells that polarized and crawled until they finally crossed the endothelial cell monolayer. “Detachment”: T cells that detached during the evaluation period. “Stationary”: T cells that did not polarize and remained stationary. 2A: Experiment imaged with 10x objective. A total of 64 cells were categorized. 2B: Experiment imaged with 20x objective. A total of 37 cells were categorized.