Table 5 Checklist before starting the experiments
From: The brain slice method for studying drug distribution in the CNS
aECF | Stock aECF |
280 mM CaCl2 | |
400 mM ascorbic acid | |
Dispensed the day before 1 L MQ water | |
Oxygen supply | |
pH meter | |
Apparatus | Orbital shaking incubator |
Vibratome | |
Nitrogen evaporator | |
Ultrasonic bath | |
Ultrasonic processor | |
Centrifuge | |
Water bath | |
ELISA reader | |
Glass- and lab-ware | Petri dishes |
45 mm high, 80 mm diameter, flat-bottomed glass beakers | |
Custom-fabricated lids of Teflon FEP film for beakers | |
Scintillation vials | |
Surgical instruments for dissection of brain | |
Ice buckets | |
Pre-labeled Eppendorf tubes | |
Nunc 96-well plates | |
Blank brain homogenate (in 4 volumes (w/v) of aECF) | |
Miscellaneous | Cyanoacrylate glue |
Cytotoxicity detection kit |