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Table 4 Preparation of samples for assessment of viability of brain slices based on the activity of released lactate dehydrogenase

From: The brain slice method for studying drug distribution in the CNS

 

aECF buffer

Low control

Sample

High control

Reaction mixture*

Background

100 μl

   

100 μl

Low control

 

100 μl

  

100 μl

Sample

  

100 μl

 

100 μl

High control

   

100 μl

100 μl

  1. * The reaction mixture should be prepared ex tempore according to the manufacturer's instructions.
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Fluids and Barriers of the CNS

ISSN: 2045-8118