Table 1 Composition of the working aECF solution
From: The brain slice method for studying drug distribution in the CNS
Weight (g)/volume (ml) | |
|---|---|
Glucose | 1.802 g |
Milli-Q water* | 600 ml |
Stock aECF | 100 ml |
280 mM CaCl2 | 5 ml |
400 mM Ascorbic acid | 1 ml |
pH (23°C) | Adjust pH to 7.6 (up to 1.5 ml of 10 M NaOH) |
Milli-Q water | Adjust volume to 1000 ml |