Western blot analyses for myelin basic protein (MBP). a. Western blots for MBP. Ten-lane precast gels were used. Each lane had an individual 5 μl sample of CSF from the categories of patients shown. After protein separation using SDS PAGE they were blotted onto a PVDF membrane for probing with antibodies. ECL detection was used to visualise the labelled bands and for semi-quantitative analysis using Image-J software. A single 40 KDa band was labelled using the antibody to myelin basic protein and only detected in PHH and SB/HC samples as shown in this representative gel. All samples were run a minimum of three times with a total number of samples as given for semi-quantitative analysis. Patient groups as for Figure 1. b. Semi-quantitative analysis of the 40 KDa band labelled with anti-MBP antibody. MBP was increased significantly (P < 0.001) in PHH and SB/HC compared to undetectable levels in normal, FOH and LOH infants. Values are Mean ± SEM of Normal n = 4, FOH n = 4, LOH n = 4, PHH n = 4, SB/HC n = 4. Values in PHH are SB/HC are significantly different to the undetectable level in normal, so effectively from the zero protein level, P < 0.001 indicated by asterisks ***.