Table 2 Summary of maximal TEER values recorded real-time by the CellZscope system for hBMEC mono-cultures using a range of different culture conditions

Cell seeding density: 3.0 × 10⁴ cells/cm², coating: collagen type I (10 μg/cm²)

3.0 × 10⁴

1.72*

25.9 (± 0.635)

Cell seeding density: 4.5 × 10⁴ cells/cm², coating: collagen type I (10 μg/cm²)

4.5 × 10⁴

3.19

43.6 (± 3.89)

Growth medium with additional HC (500 nM) for 3 days (only apical), coating: collagen type I (10 μg/cm²)

4.5 × 10⁴

3.19

43.2 (± 4.27)

Growth medium with additional HC (1500 nM) for 3 days (only apical), coating: collagen type I (10 μg/cm²)

4.5 × 10⁴

3.19

40.4 (± 0.646)

Cell seeding density: 6.0 × 10⁴ cells/cm², coating: collagen type I (10 μg/cm²)

6.0 × 10⁴

1.72*

36.3 (± 2.06)

Cell seeding density: 9.0 × 10⁴ cells/cm², coating: collagen type I (10 μg/cm²)

9.0 × 10⁴

4.15

38.9 (± 0.928)

Growth medium with dexamethasone (500 nM) for 3 days (only apical), coating: collagen type I (10 μg/cm²)

9.0 × 10⁴

4.15

35.9 (± 0.338)

Growth medium with dexamethasone (1000 nM) for 3 days (only apical), coating: collagen type I (10 μg/cm²)

9.0 × 10⁴

4.15

38.0 (± 1.26)

Growth medium with additional hEGF (50 ng/mL) for 3 days (only apical), coating: collagen type I (10 μg/cm²)

9.0 × 10⁴

4.15

36.4 (± 0.902)

Growth medium with additional hEGF (100 ng/mL) for 3 days (only apical), coating: collagen type I (10 μg/cm²)

9.0 × 10⁴

4.15

40.0 (± 1.94)

Growth medium with 8-(4-CPT)cAMP (250 μM) and RO-20-1724 (17.5 μM) (apical and basolateral), coating: collagen type I (10 μg/cm²)

9.0 × 10⁴

2.84

20.0 (± 0.509)

Growth medium with 8-(4-CPT)cAMP (250 μM) and RO-20-1724 (17.5 μM), added after 2 days in vitro (apical and basolateral), coating: collagen type I (10 μg/cm²)

9.0 × 10⁴

2.84

20.9 (± 1.36)

Cell seeding density: 15 × 10⁴ cells/cm², coating: collagen type I (10 μg/cm²)

15 × 10⁴

2.75

28.4 (± 2.47)

Coating: collagen type IV/fibronectin (80/20 μg/cm²)

15 × 10⁴

2.75

29.3 (± 1.10)

Coating: matrigel (80 μg/cm²)

15 × 10⁴

2.75

16.6 (± 0.183)

Growth medium with 10% HS (apical and basolateral), coating: collagen type I (10 μg/cm²)

15 × 10⁴

1.26*

20.4 (± 0.867)

Growth medium with 20% HS (apical and basolateral), coating: collagen type I (10 μg/cm²)

15 × 10⁴

1.21*

26.2 (± 0.324)

ACM-1 (apical and basolateral), coating: collagen type I (10 μg/cm²)

15 × 10⁴

1.64*

13.5 (± 0.614)

Mixture of ACM-2 and growth medium 20% FBS (1:1) (apical and basolateral), coating: collagen type I (10 μg/cm²)

15 × 10⁴

3.35

23.8 (± 0.572)