Mean TEER values for hBMEC cell line in mono-cultures and co-cultures with immortalized astrocytes (SVG-A) and immortalized pericytes (HBPCT). Experiments using 24-well tissue culture inserts from Corning® (transparent PES membrane, 0.4 μm pore size, 4.0 × 106 pores/cm2, n = 2). Maximal TEER values (30.7 ± 0.660 Ωcm2 on day 12) were obtained with hBMEC mono-cultures. Co-culture models with SVG-A and HBPCT cells did not result in increased TEER values. Data were recorded with an EVOM coupled to an Endohm-6 measurement chamber.