Skip to main content


Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Figure 5 | Fluids and Barriers of the CNS

Figure 5

From: Comparative study of four immortalized human brain capillary endothelial cell lines, hCMEC/D3, hBMEC, TY10, and BB19, and optimization of culture conditions, for an in vitro blood–brain barrier model for drug permeability studies

Figure 5

Immunofluorescence staining of tight junction protein ZO-1 in hBMEC cell line. hBMEC cells were grown on collagen-coated glass coverslips for 48 h followed by paraformaldehyde fixation. Even with a high background noise level especially on nuclei, ZO-1 was detected at the leading edge of migrating hBMEC cells (see white arrows). Scale bar: 30 μm.

Back to article page