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Figure 5 | Fluids and Barriers of the CNS

Figure 5

From: Comparative study of four immortalized human brain capillary endothelial cell lines, hCMEC/D3, hBMEC, TY10, and BB19, and optimization of culture conditions, for an in vitro blood–brain barrier model for drug permeability studies

Figure 5

Immunofluorescence staining of tight junction protein ZO-1 in hBMEC cell line. hBMEC cells were grown on collagen-coated glass coverslips for 48 h followed by paraformaldehyde fixation. Even with a high background noise level especially on nuclei, ZO-1 was detected at the leading edge of migrating hBMEC cells (see white arrows). Scale bar: 30 μm.

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