Figure 3
From: Genetic mouse models to study blood–brain barrier development and function
Abcb1a / Abcb1b / Bcrp triple knockout mice have a leaky BBB to Rhodamine123. Adult wild type (C) or Abcb1a/Abcb1b/Bcrp triple knockout mice (3KO) were given an intravenous injection of Rhodamine123 (10 ng). After 1 hour the blood compartment was removed by transcardiac perfusion with PBS, the brains were removed, hemisected down the midline and imaged for Rhodamine123 fluorescence with a Fujifilm imager LAS 4000. Figure 3 represents an image of the hemisected brains with the cerebellum at the top of the image and frontal cortex towards the bottom of the image. More Rhodamine123 (dark color) was observed in Abcb1a/Abcb1b/Bcrp triple knockout mice compared with littermate controls. To demonstrate the consistency of the phenotype between different animals, 3 wild-type and 3 Abcb1a/Abcb1b/Bcrp triple knockout mice brains were utilized.